Tissue Microarrays plays a major role in order to conduct a high throughput analysis regarding a large number of tissue samples. And therefore it is being used on a large scale. These samples are actually collected as well as archived following the paraffin blocks/formalin. Earlier, paraffin blocks have only been used to analyze and study the histological sample from cancer/tumor infected tissues. But Tissue Microarrays has brought a great change and make the study of histological samples quite easier. With Tissue Microarrays, it becomes easy to have all the needed information on a single slide. First, a paraffin block with an array is prepared and then samples from the histological samples blocks to paraffin block is transferred using a needle. To get more information on tissue microarray topic visit https://www.biochain.com/general/the-tissue-microarray-tissue-analysis-from-research-to-treatment.
Tissue Microarray and DNA Microarrays –
Moreover, Tissues microarrays are completely different from DNA microarrays. To put in easy words, Tissue Microarrays come up with each spot on an array indicating towards a cloned cDNA or oligonucleotide aimed to churn out sequence. Each array holds patient-oriented particular histological samples from cancer infected tissues. Needless to say that tissues microarray technique is considered an ideal one to do the screening of a genetic marker or protein all across the thousands of samples. On the other hand, DNA microarray is ideal to study gene expression all across thousands of genes.
Needless to say that Tissue Microarrays has made possible to go with an ideal method to achieve concurrent information from a multitude of tissues. Using a single paraffin block, representative parts of tissues can easily be assembled to study and analysis in respect of protein expression patterns. Moreover, digital scanning plays a major role to generate corresponding pictures to understand in a better way and for distributing data easily.
Advantage Of Using TMA Technology
The prominent benefit of going with Tissue Microarray technology is that a large amount of data can easily be obtained following a single immunostaining protocol. This technique helps to avoid experimental variability which sometimes also takes extra time and efforts. It means the only a particular amount of scarce tissue will be required allowing for the study of large patient cohorts. It may sound a bit bizarre but true that a total number of 250 consecutive sections have a thickness of 4um can easily be cut from a Tissue Microarray block to use for immunohistochemical. It makes easier to decide particular protein expression patterns for 250 different antibodies.
It is also quite good because of imparting the facility of doing the wider analysis. It means samples from 1000 patients can be analyzed following this method. Moreover, the best thing is that TMA technology is regarded quite cost-effective technique. To put in simple words, tissue microarrays about doing a typical cohort analysis needs fewer reagents as well as stains at the time of enabling more assays.
TMA method has brought a great change to the medical field because of catering a wide array of facilities and making easier for the pathologist to do the analysis. This method holds several other benefits over using the conventional one. TMA makes easier examining tissue from many patients following the same slide.